I Have A Super USB Drive

half a month later.

Chen Chen stopped the instrument in his hand and stretched out in front of the 100-grade ultra-clean workbench, a look of excitement flashing in his eyes.

"The fertilized egg of clone 30 has been induced and can be implanted into the mother."

Chen Chen began to fill in the experimental records as he stated. The experimental recorder on his head faithfully recorded every frame of the picture. These records will become the most valuable data in the future.

At this time, the entire experiment was still in the early stage, that is, the cloning stage. Chen Chen needed to use a micromanipulator to remove the nucleus from a total of thirty-six egg cells, and implant another nucleus to form a cloned fertilized egg.

This is also commonly known as "somatic cell nuclear transfer reprogramming".

The road to cloning technology has not been smooth. From 1938, when German embryologists first proposed the concept of cloning, to 1996 when Dolly the cloned sheep was born, and in 2012, humans discovered that caffeine can increase the success rate of reprogramming of primate egg cells. , Human cloning technology has been developed for nearly a century.

Until today, cloning technology has matured and commercialized. Many people are willing to spend a lot of money to clone deceased pets, and the emergence of profitable pet cloning companies is enough to show that cloning technology has become increasingly mature.

Because of this, Chen Chen was able to process dozens of SD rat cloned fertilized eggs in half a month.

However, mature technology does not mean simple.

What is clone?

There is such a fable in the West:

It is said that there is a country, and there is a poisonous spring in the country. Anyone who takes a sip will go crazy and become a lunatic.

All the subjects of this country drank this spring water and fell into a state of madness, but the king did not drink it, so he did not go mad with it.

As a result, the madmen all over the country felt that the king had a mental problem, so they tied the king up and tried to cure his madness.

In the end, the king couldn't stand the torture, so he could only drink the spring water and go crazy with the people...

And cloning is the true reflection of this fable.

In a cell, there are dozens of substances such as cell membrane, cytoplasm, organelles, and nucleus, and the most important one is the nucleus, which is an organ that contains all the genetic information in the cell.

Generally speaking, if a cell wants to carry out activities, it must first declare to the nucleus. After the nucleus approves, it will call the relevant gene, and then let a molecule called messenger RNA carry the genetic information out, so that the relevant organelle can execute its own function. Order.

It can be said that in the cell, the nucleus is the king, and the other structures and components in the cell are the subjects.

But if one day, scientists replace the king of the nucleus, especially the egg cell, human beings will remove the nucleus of the egg cell and insert the nucleus of the somatic cell of the object to be cloned into the egg cell instead.

Then, scientists use electric current and chemical agents to change the characteristics of this egg cell and turn it into a fertilized egg, just like making the whole country drink poisonous spring water, so the scene in the fable happened.

The subjects asked the nucleus to issue an order to let the fertilized egg develop, but the newly appointed nucleus refused. After all, he is not the nucleus of the egg cell. If you can't let me develop the fertilized egg, I will develop it. The nucleus of my somatic cell is a mutation. It becomes cancerous, swallowed by white blood cells, and never develops!

However, with the constant pressure of the subjects, this somatic cell nucleus finally felt "outrageous" and had to change its position and transform itself into an egg cell nucleus.

At this point, the egg cell successfully develops into an embryo, which will be implanted into a mother's uterus, and a cloned life will eventually be born.

And this life has the exact same gene of the owner of the nucleus flowing in its body.

Cloning succeeded!

certainly,

In practice, the difficulty is still huge. Not to mention the damage of those chemicals, just exchanging the nucleus of the egg cell is equivalent to a major operation.

After all, the egg cell needs to remove the nucleus first, and then implant the allogeneic cell nucleus. It is already considered that the egg cell has a big life without being killed on the spot.

It was Chen Chen who took NZT48 to maintain such a high success rate.

At this moment, the door behind Chen Chen was knocked, and Wang Wei's voice came in.

"Chenzi, the second batch of clones was successfully born, and no hidden disease has been found for the time being."

Different from Chen Chen's full of energy, Wang Wei's voice carried an undisguised tiredness.

"Okay, I'll be right over here."

Chen Chen stood up and started to clean up the workbench in front of him. After freezing a few precious cloned fertilized eggs, he rushed to another room.

As soon as he entered the door, Chen Chen heard a squeak, which was the cry of a few newly born mice, while Wang Wei and Xia Yin were sitting in front of the cage, silently observing.

The newborn rat was red all over, with only two black spots the size of sesame seeds in the eye area, squeaking like red worms.

"Get ready to start, extract neural stem cells to make cell suspensions."

"it is good."

Wang Wei nodded. After working together for a month, Wang Wei was completely convinced by Chen Chen's skills and perseverance. If it wasn't for Chen Chen, no student in the entire Academy of Biosciences could have achieved this step.

Xia Yin, who was on the side, opened her mouth and looked at a bunch of newborn mice who didn't even open their eyes, showing hesitation, "Are we really going to do it now?"

"Since you have chosen this line of work, don't be sympathetic." Chen Chen said lightly, "As long as you have respect for these animals who have dedicated their lives to science, it is enough."

"..." Xia Yin was silent.

Immediately, the three began to compare the genes of the cloned mouse and the original owner, and after confirming that there was no error, they were executed.

The poor newborn rat lost his life completely before he opened his eyes to see the world.

"Is the ice PBS dish ready?" Chen Chen asked.

"Here." Xia Yin carefully brought a few petri dishes and looked at Wang Wei, "It's time to start."

"it is good."

Wang Wei took the petri dish and started to kill the rat offspring on the operating table, and took out the brain tissue from the rat offspring's corpse.

He then placed the rat's brain in an ice-cold PBS dish, skillfully isolated the hippocampus, put it in the buffer, and then, like an assembly line, turned around again to process the second rat.

Chen Chen walked to the position in front of Wang Wei, cut the hippocampus into fine pieces, added 0.25% pancreatin at the same time, and put it into a 37-degree incubator. He said to Xia Yin, "Is the planting solution ready?"

"It's ready." Xia Yin took the test tube from the shelf.

Chen Chen nodded and turned around to operate the second petri dish that Wang Wei had handled.

Xia Yin waited for two minutes, then took out the petri dish from the incubator, put it into the centrifuge for separation, and then added the planting solution and blowing to form a cell suspension.

"Drip drip!"

At this moment, the electronic alarm clock in the laboratory rang, and it was nine o'clock in the evening.

"Okay, that's it for today's experiment."

Although there was still work to be done, Chen Chen didn't mean to stop at all, he put down the tools in his hand, packed up his things, turned around and walked out of the laboratory.

"But we're still half an hour away from finishing today's work?" Wang Wei asked in confusion.

"Work and rest is the most important thing." Chen Chen shook his head and said solemnly, "If you want to do this job for a long time, you can't do it without a good body."

Watching Chen Chen leave, Wang Wei and Xia Yin looked at each other,

"Chen Chen is really strange."

Xia Yin complained, "At work, you race against time and you are full of energy. One person can do the workload of several people, but when it comes to his own set off-get off work time, he doesn't want to work for an extra second..."

"Maybe this is the quirk of scientists?"

Wang Wei shook his head, "Let's clean up as well and do the rest tomorrow."

"it is good."

Xia Yin nodded, and the two left the laboratory one after another.