Supreme Pharmacist System

Wu Yan knew that Zhou Wen must have a special extraction technique.

But seeing that he was unwilling to say it, she didn't force it, after all, it was someone else's housekeeping skill, and it would be harmful and useless to leak it out.

Afterwards, the two of them talked about the remuneration again. Wu Yan told Zhou Wen again what she said to Shi Lei before, and finally asked with a smile, "Do you have any questions?"

Zhou Wen thought of the task and said, "Can I ask, am I a contract worker or a regular worker?"

"Well..." Wu Yan hesitated for a moment, she didn't expect Zhou Wen to ask this question.

What's going on with biology now, she believes that Zhou Wen should be very clear in his heart, the contract of Jiangzhou First People's Hospital is so generous, let alone a small undergraduate student, even master's and doctoral students in biology, many people It is also a wish.

It's really too much for him to go to the sky and solve the staffing problem directly. She herself is still a contract worker until now.

Wu Yan thought for a while and said, "Generally speaking, if you are hired by the hospital, it must be a regular worker, but you are an employment contract for the PCR team of the testing center, so I'm afraid you can only be a contract worker."

"Contract workers..." Zhou Wen hesitated.

In fact, he really doesn't care if the contract worker is still a regular worker. As long as he can go to the testing center for an internship, he is willing to work even as a temporary worker.

Isn't this task requiring him to become a regular worker within a week? He had to stretch a little bit.

1000 experience points and 1000 points, and a chance to open a black iron box.

Wu Yan originally thought that the matter had already been settled, but she didn't expect that there would be another problem here, so she said earnestly: "Although you are a contract worker, the salary is calculated according to the regular worker, and there is no difference.

In addition, everything is done step by step. You go to work first, and it won’t be too late to solve the compilation problem after a while. "

Zhou Wen asked with a smile: "You are my senior, and you have also been in the hospital for a year. To be honest, if I really sign a contract worker, when will the establishment be resolved?"

"Well..." Wu Yan was a little embarrassed, she picked up her coffee cup to cover it up.

After putting down the cup, Wu Yan said: "Well then, I'll help you go back and ask. But to be honest, don't expect too much."

Zhou Wen nodded and said with a smile, "Okay, then I'll trouble Senior Sister."

"You're welcome."

Zhou Wen was still centrifuging, so he didn't wait too long, and after a few polite words, he got up and left.

...

When they returned to the laboratory, Yang Yudong and Gu Hongyun couldn't wait to ask him if he had a successful interview?

Zhou Wen said with a smile: "I made a condition. She said that she would go back and discuss with the leader, and she would give me a letter after the discussion."

Yang Yudong said speechlessly: "The conditions are so superior, do you still want to raise conditions?"

Zhou Wen said: "I just want to try their reserve price because I see the opportunity is rare."

Yang Yudong: "..."

He really wanted to use the extraction gun to pull out Zhou Wen's brain a little, to see what was going on in his mind all day long?

Zhou Wen giggled and went to do the PCR experiment.

The full name of PCR is Polymerase Chain.

The Chinese name is "polymerase chain reaction", also known as "polymerase chain reaction", which is an in vitro nucleic acid amplification technology developed in the mid-1980s.

In a test tube, the target gene or a certain DNA fragment to be studied can be amplified to 100,000 or even a million times within a few hours.

Its principle is basically similar to DNA replication in human cells, but the reaction system is relatively simple.

Of course, this does not mean that PCR is easy.

On the contrary, PCR is very difficult.

First, through high temperature "denaturation", a piece of double-stranded DNA is melted and turned into two single-stranded DNA.

After "annealing", the "primer" and the DNA as a template are complementary combined at low temperature to form a partial double strand.

Finally, "extend" in the middle of the fire, and the DNA polymerase catalyzes the extension reaction of the DNA strand starting from the primer to become two double-stranded DNA.

Then keep repeating this process.

1 becomes 2, 2 becomes 4, 4 becomes 8, 8 becomes 16, 16 becomes 32...

Through PCR technology, a sufficient amount of DNA can be amplified from a hair, a drop of blood, or even a cell for analysis, research, detection and identification.

such as paternity testing;

such as vaccine research;

such as the prevention and treatment of viral diseases;

Or the study of genetic diseases, etc., require a lot of PCR.

Of course, there is no need to do PCR for the rice aphid biochemical reagent experiment that Zhou Wen did today.

Aphids are very low-level insects. Generally, drugs are used to act on them directly, and then it is enough to look at their genetic characteristics.

The reason why I still need to do PCR is more to learn PCR.

The PCR reaction system includes water, buffer, DNTP (deoxyribonucleoside triphosphate), primers P1/P2/DNA template and TAQ enzyme.

After everything was ready, Zhou Wen adjusted the volume of the extraction gun, and then extracted 30 μl of water into three sets of PCR tubes.

Strictly follow the operating procedures when absorbing liquid, absorb liquid vertically, absorb slowly and release slowly.

After changing the pipette tip, extract 5 ml of the buffer into a PCR tube.

Add DNTP/P1/P2/DNA templates to the tubes respectively...

Thanks to the study during this period, except for being a little nervous at the beginning, Zhou Wen did it in an orderly manner.

After the required raw materials have been added, the next step is centrifugation.

After setting the speed as required, waited for three minutes, and the centrifugation was completed.

Zhou Wen took out the PCR tube containing the mixture from the centrifuge, and immediately put it on the PCR machine for amplification.

Set 94°C for pre-denaturation for 4 minutes.

A 20-cycle amplification phase was then set.

High temperature, low temperature, medium temperature, high temperature...

1 becomes 2, 2 becomes 4, 4 becomes 8, 8 becomes 16, 16 becomes 32...

Zhou Wen waited anxiously, feeling like a husband outside the delivery room, silently praying "mother and child are safe", and at the same time thinking, I don't know if the baby will be a boy or a girl? Healthy or not?

At this moment, Zhou Wen suddenly thought that the system points can speed up the progress of the experiment. Instead of waiting here for a few hours, how about speeding up?

Anyway, no one here saw it.

Thinking of this, Zhou Wen immediately summoned the personal attribute page, and after clicking the points, an option popped up in front of him.

【Would you like to consume points to speed up the progress of the experiment? whether】

【Yes】.

【This mission needs to consume a total of 20 points, please confirm again】

Seeing that Zhou Wen could speed up the progress of the experiment with only 20 points, there was no reason to hesitate, so he immediately chose to confirm.

Before Zhou Wen could react, the next second, the PCR machine had completed the amplification.

Zhou Wen was amazed in his heart, the power of the system was simply mysterious.

With a sigh in his heart, he soon became nervous again.

It's the first time to do PCR, I don't know if it can be successful?

Zhou Wen opened the PCR machine and took out the PCR tube from inside.

Originally, the following was product analysis, but Zhou Wen had eyes of true sight, and he narrowed his eyes slightly, and soon he saw the high-density double-helix DNA.

"perfect~"

If it weren't for wearing a mask, Zhou Wen would have laughed out loud.

Anyone who studies biology counts as one. Who didn't learn to do PCR after countless failures? Is there anyone who can do amplification for the first time like him and succeed at the first time?

And the amplification effect was unexpectedly good, it was perfect.

...

Jiangzhou City, First People's Hospital, Testing Center, PCR Group.

Wu Yan returned to the hospital after having lunch outside, and immediately went to the team leader's office to report the situation.

"Where are people?" Zhang Shuguang, who was writing the experiment report, asked without raising his head.

Wu Yan said with some embarrassment: "That...hasn't come yet."

When she went, she patted her chest and promised that she would be able to grab it, but the cowhide blew up.

Zhang Shuguang raised his head and said in surprise, "Why, you don't want to come?"

Wu Yan shook her head and said, "No, it's just that he said..."

Wu Yan told Zhang Shuguang about Zhou Wen's request.

Zhang Shuguang tapped the pen on the desk with his left hand, and said with a smile: "You say, you, such a smart person, why did you lose the chain at a critical moment?"

Wu Yan asked strangely: "Why did I lose the chain? He wants to be paid a formal job, how dare I go overboard and promise for you?"

Zhang Shuguang said with a smile: "What's the matter with Yue Zu acting as a substitute? He is a biology undergraduate student. He dares to ask you for a career salary. Don't you think about why?"

"Why?" Wu Yan was puzzled, and quickly realized that she was surprised: "Oh~~~You mean..."

Zhang Shuguang laughed and said, "You've already come to find him. As long as it's not too outrageous, you can promise him first, and talk about it later if you have any questions. Verbal promises are not guaranteed, right?"

"Hmm~ Boss, I know I was wrong." Wu Yan nodded again and again, admitting the mistake, "Then I should call him now?"

Zhang Shuguang shook his head amusedly, "I'm back now, what's the rush, I'm afraid he won't be able to run away. Anyway, we are also in Jiangzhou No. 1 hospital, so we can't be underestimated. Stretch him for two days before we talk about it."